Matters needing attention in calling the citizen service hotline:
1. The facts of the call complaint must be clear, leaving your real name and effective contact information;
2. If the complaint involves the scope of Party committees, people's congresses, CPPCC, military and judicial organs, citizens should report directly to the complaint departments of these units;
3. If the complaint has entered the judicial process (the court has made a second trial or a judgment), the citizens should directly report it to the Standing Committee of the Municipal People's Congress, the Political and Legal Committee of the Municipal Party Committee or the judicial organ at the next higher level; For emergency help involving the safety of life and property, citizens should call 1 10, 1 19 directly.
4. Parents should strengthen the education and management of minors to prevent misdialing and mistaking; For invalid calls, the agent will patiently persuade and persuade;
5. For malicious harassing calls, the public security department will press "? Should the relevant provisions of the Public Security Administration Punishment Law be investigated for responsibility? .
Main acceptance
A * * * departments and staff responsibilities, procedures, administrative examination and approval, administrative acceptance, investment and other policies and regulations;
Two, opinions and suggestions on the city's reform and opening up, economic construction, urban construction and management;
Three. Needs to be solved in social life such as labor security, medical services, education and safety;
Four. The needs that need to be solved in the production, operation and development environment of the enterprise;
V. Criticism and complaints about the * * * department and its staff.
What are the precautions for hot air balloons? Hot air balloon has a history of more than 200 years abroad, but it started late in China. At present, there are only 120 hot air balloons in China. The cost of hot air balloon is relatively high, each balloon is about 80,000 yuan to 65,438+10,000 yuan. To drive a hot air balloon, you must first obtain a hot air balloon driving license issued by the Civil Aviation Administration of China. Each flight not only needs the cooperation of several ground crew members, but also needs to apply to the civil aviation management department and the air force command department for filing in advance. Even if you just go to the club to "experience", you have to fly to 600 yuan for 30 minutes at a time, which is more expensive than playing golf.
At present, in order to get the qualification to drive a hot air balloon in China, the tuition fee needs about 16000- 18000 yuan. After the completion of the course, if you still fly in the professional club, you still have to pay a certain flight fee for each flight; The cost of experiencing a flight is about 400-500 yuan per hour; If you want to buy a hot air balloon and engage in flight activities in your own area, you need to go to the air traffic control department for approval and declaration of flight airspace. It costs about 76 thousand yuan to buy a standard hot air balloon. In some tourist attractions in China, there is also a kind of "tethered flight", that is, a hot air balloon that does not fly away. Although it can only rise to the height of 30 to 50 meters, it can still initially experience the feeling of "ruling the world" when hot air balloons fly.
Precautions:
1. In order to prevent fire, pilots must wear cotton cloth, not skirts, high heels, sandals, etc. But you must wear sports shoes. In order to prevent burns, you must wear trousers and a cotton baseball cap.
2, hypertension, heart disease patients can not carry out hot air balloon exercise.
The best time for hot air balloon flight is within two hours after sunrise or two hours before sunset, because the airflow is the most stable at this time. The windy season is not suitable for flying hot air balloons.
4. Every flight has a certain flight cost, basically from 400 yuan to 500 yuan every hour.
The service life of hot air balloon is basically 9 years, so annual inspection and sequential inspection must be carried out.
6, high altitude flight should pay attention to cold, every 1000 meters, the temperature dropped by 6.5 degrees.
7. Professional flight requires a driver's license issued by CAAC.
8. In addition to the necessary equipment, you need to wear GPS, electronic compass, walkie-talkie, tool knife, ZIPPO lighter, etc.
What are the precautions for RT-PCR? Some good habits in the experiment
Before adding the reagent, mix it evenly to avoid uneven concentration after standing for a long time.
After the pipette is used up, it should be put back to the maximum metering position to prevent the spring from losing its elasticity over time.
Remember to close the water bath box, remember to remember.
Discuss with you more and pay more attention to the experience discussed by others, which is almost the fastest shortcut to promotion.
All reagents are prepared by ourselves. If you have any questions, you can find out the reasons.
Measures to prevent ribonuclease pollution
1. All glassware should be dried at 180℃ for 6 hours or more before use.
2. Plastic utensils can be soaked in 0. 1% DEPC water or washed with chloroform (note: plexiglass utensils cannot be used because they will be corroded by chloroform).
3. The electrophoresis tank of plexiglass can be washed with detergent, washed with double distilled water, dried with ethanol, then soaked with 3% H2O2 at room temperature 10min, washed with 0. 1% DEPC water and dried.
4. The prepared solution should be treated with 0.65438±0% DEPC at 37℃ for more than 65438 02 hours. Then the residual DEPC is removed by autoclaving. Reagents that cannot be autoclaved should be prepared with sterile double distilled water treated by DEPC, and then filtered and sterilized with 0.22μm filter membrane.
5. Operators should wear disposable masks, hats and gloves, and change gloves frequently during the experiment.
6. Set up a special RNA operation laboratory, and all instruments should be dedicated.
Second, the commonly used ribonuclease inhibitors
1. Diethyl pyrophosphate (DEPC): It is a strong but incomplete ribonuclease inhibitor. Protein is denatured by binding with imidazole ring of histidine, the active group of ribonuclease, thus inhibiting the activity of ribonuclease.
2. Guanidine isothiocyanate: At present, it is considered as the most effective RNase inhibitor, which not only lyses tissues, but also inactivates RNase. It can not only destroy the cell structure and dissociate nucleic acid from nucleoprotein, but also has a strong denaturing effect on ribonuclease.
3. Vanadium oxide ribonucleoside complex: a complex formed by vanadium oxide ions and nucleosides, which combines with ribonuclease to form a transition substance, can almost completely inhibit the activity of ribonuclease.
4. Protein inhibitor of ribonuclease: acidic glycoprotein extracted from rat liver or human placenta. Ribonuclease is a non-competitive inhibitory ribonuclease, which can be combined with various ribonucleases to inactivate it.
5. Others: SDS, urea, diatomite, etc. It also has a certain inhibitory effect on ribonuclease.
Because DEPc has no difference in the modification of protein and RNA, it can not be used in the process of separating and purifying RNA, and it is not compatible with some buffers (such as Tri).
In all RNA experiments, the most critical factor is the isolation of full-length RNA. The main reason for the failure of the experiment is the pollution of ribonuclease.
RNase can tolerate various treatments without inactivation, such as boiling and autoclaving.
The main potential source of pollution caused by researchers is their hands. Therefore, Qin Ying changed his gloves when doing RNA experiments.
What are the precautions for wide-mouth bottles and narrow-mouth bottles, and the use and precautions of funnels? Narrow mouth bottle
A glass container for storing liquid reagents, so the mouth is relatively small. There are two kinds: transparent and brown. Brown bottles are used to hold reagents that need to be kept away from light.
Precautions for use: it cannot be used for heating. When using the reagent, the bottle stopper should be inverted on the table, plugged tightly after use and sealed if necessary. Because the inside of the bottle mouth is frosted, with the glass frosted plug, it can't hold the strong alkaline reagent. If alkaline reagents are installed, please use rubber plugs instead.
wild-mouth bottle
A jar is a glass container for solid reagents, which is transparent and brown. Brown bottles are used to hold reagents that need to be stored away from light.
Precautions for use: it cannot be used for heating. When using the reagent, the bottle stopper should be inverted on the table, plugged tightly after use and sealed if necessary. Because the inside of the bottle mouth is frosted and equipped with a glass frosted plug, a jar with a glass plug cannot contain a strong alkaline reagent. If alkaline reagents are installed, please use rubber plugs instead.
What are the precautions for loans? The loan amount through China Merchants Bank is small (less than 50,000). If you are already a user of China Merchants Bank and downloaded the mobile banking APP of China Merchants Bank, you can try to apply through this interface by logging in to mobile banking and clicking My-All-Loan-I want a loan. If you meet the credit card requirements of China Merchants Bank, you can try to apply for a credit card.
The loan amount is more than 50,000 yuan. If there is China Merchants Bank in your city, you can try to apply for a loan through China Merchants Bank outlets. Due to the different requirements and application materials of each loan project, the current loan purposes are: car purchase, parking, decoration, education, bulk consumption shopping, tourism and other legitimate personal or family consumption; And the use of business turnover. You can call 95555 at 8:30- 18:00 to enter the manual service and provide the loan purpose and city for detailed information. Whether the loan application is approved or not shall be subject to the comprehensive audit results of the personal loan department of the handling bank.
What are the precautions for soldering? 1. Don't set the temperature of the tin furnace too high to avoid the accelerated oxidation of the tin surface. Generally set at about 300 degrees or according to the actual use needs;
2. First, put an appropriate amount of tin bars in the tin can, turn on the power supply, turn on the power switch, adjust the temperature to about 250 degrees, and tin the red heating tube with tin bars until the tin surface covers the heating tube. When tin bars begin to melt, tin bars should be added in time until the molten tin surface reaches a suitable height. When there is no solder in the tin furnace, do not electrify and heat it.
3. Temperature range of general tin furnace: 0-500 degrees. But the actual maximum temperature is about 300-400 degrees;
Matters needing attention in the use of tin furnace
A. The tin furnace is connected with a grounding wire, and the user must connect it well, and ensure good grounding to ensure safety;
B. Before using the tin furnace, check whether the power supply voltage is consistent;
C. The tin furnace should be kept dry, and don't work in wet or rainy environment;
D the tin furnace should be placed stably, and flammable items and other items should not be placed within 0.5m around it;
E. Operators should use goggles and heat-resistant gloves, and pay attention to avoid foreign objects falling into the tin melting pot during use to prevent accidents;
F. It is forbidden to move after electrifying, and it is forbidden to knock, disassemble and install its electric heating parts at will;
G when in use, the temperature of the shell is 50℃-80℃, which is a normal phenomenon. Pay attention to the high temperature and do not touch the shell;
H turn off the power supply after use, and do not turn on the power supply for heating without supervision;
First, if there is any fault, please check and eliminate it by professional maintenance personnel.
What are the precautions for blood transfusion? (1) Before blood transfusion, the appearance of whole blood must be strictly checked, and the blood bag must be checked for damage and leakage, and the blood color is qualified; It is also necessary to carefully check whether there are any errors in patients, cross-matching reports and blood to be transfused, including the names, genders, ABO and Rho(D) blood types of patients and blood donors, the results of cross-matching tests and antiglobulin tests, the number of blood bags, blood types and blood volume, etc. Before blood transfusion, it should be checked by two people. (2) Check the medical record number, patient name, blood type, etc. Before blood transfusion, then use a standard blood transfusion device with a filter (the pore size of the filter is about 170μ m, and the total effective filtering area is 24-34cm2, which can filter out aggregated platelets, leukocytes and fibrin that may exist in blood and blood component products). (3) Take the blood out of the refrigerator before transfusion and leave it at room temperature for no more than 30 minutes. Before transfusion, gently mix the blood in the blood bag to avoid severe shock. Other drugs should not be added to the blood. If dilution is needed, only intravenous saline can be used. (4) Wash the blood transfusion pipeline with normal saline before and after blood transfusion. When different blood donors transfuse blood continuously, after the first bag of blood is transfused, normal saline is injected intravenously to flush the infusion set, and then the next bag of blood is continuously transfused. (5) The blood transfusion process should be slow first and then fast, and then the infusion speed should be adjusted according to the condition and age, and the adverse reactions of the blood transfusion recipients should be closely observed, and the abnormal situation should be handled in time. During the first 10- 15 minutes of blood transfusion or during the first transfusion of 30-50 ml, medical staff must pay close attention to whether there are any adverse reactions. In case of adverse reactions, stop blood transfusion immediately, report to the responsible doctor for timely diagnosis and treatment, and notify the blood transfusion department or blood bank to investigate the necessary reasons. Normally, warm blood is not needed for blood transfusion. (6) After blood transfusion, keep the blood bag in the refrigerator at 2-8℃ for 24 hours to prevent accidents. (7) After blood transfusion, medical staff should fill in the receipt of blood transfusion reaction investigation item by item, and return it to the blood transfusion department for preservation on the second day after blood transfusion. The blood transfusion department reports statistics to the medical department (department) every month, and the doctor is responsible for recording the blood transfusion in the medical record. (8) After the blood transfusion, the medical staff will post the second copy of the blood transfusion form in the medical record.
What are the precautions for reversing? When reversing, try to point the front of the car to a place with a wide field of vision and room for manoeuvre, so as to observe the dynamic people or cars. Don't back up in places where traffic regulations don't allow it, in case of accidents.
Expand:
1, Introduction:
The behavior of a motor vehicle driving backwards is reversing. It also means that people "reverse" when traveling, that is, change from one car to another.
2. Basic elements
When you enter the parking lot, you must first visually check the width of the parking space, whether there are obstacles behind it, whether there are cars parked on both sides, and whether the length conforms to your own model. When you can't see clearly at night, you'd better come down and observe the exact position before you pour.
When reversing, try to reverse from the left (if possible). The left side is convenient for the reversing person to observe. In the process of reversing, you should look behind first, and then pay attention to the rearview mirrors on both sides. The lateral distance on the right side is greater than that on the left side. Because the turning radius of the front wheel is larger than that of the rear wheel, the front of the car will swing outward when reversing, which is easy to scrape to the right if you don't pay attention. When reversing, it is mainly idle, and the body should be corrected in time. The reference point of each car is different, and the timing of the direction is different, so we won't generalize here. [ 1]
Sometimes I stop in a straight line along the shoulder of the road, and often I have to fall to the middle of two stopped cars. This kind of reversing is difficult, so it's better to have someone to direct it. Pay attention to the lateral distance from the adjacent car when pouring. In the rearview mirror, when you see the rear of the car approaching the shoulder, hit the direction quickly. When the car body is straight, turn back quickly. Don't forget the distance from the rear car when reversing. If you can't grasp the distance, come down and have a look before you pour. In the process of reversing, the steering speed should be fast, so as to straighten the car body in a short time. Sometimes a forward or backward movement may not be able to straighten the car body, and it takes many times to park the car.
What are the precautions for shooting? The classic shooting postures are lying posture, standing posture and kneeling posture, and squatting posture can be used in special circumstances. The following is a brief introduction to an exercise, the key points of prone shooting 100 meter static target.
First, posture.
The angle between the shooter's trunk and the shooting projection is generally 10 ~ 20 degrees after the shooter is ready on the prone shooting mat. Feet should be close to the ground in the shape of an "outer figure of eight", and the left leg should be straight, which is similar to the left side of the body. When the left hand holds the gun, the angle between the left forearm and the ground should be not less than 30 degrees, and the angle between the upper arm and the ground should be kept at about 45 degrees. The butt of the gun rests on the right shoulder socket, the shoulder rests firmly on the clavicle, and the right hand holds (the gun neck). Press your right cheek tightly. When sticking to the cheek, the gravity of the head is straight down and the neck is relaxed. The whole body can only feel the force of the gun forward and downward, and there is no force of lateral push and pull.
The height of the support depends on the height of the individual and the length of the right arm, which is generally about 20-26 cm.
Second, according to the gun.
Press the gun, the right side of the body is slightly in a straight line with the gun body, the right hand holds the handle forward, the first section of the index finger rests on the trigger, and the right elbow is propped up as much as possible. Hold the magazine in your left hand, support your left elbow on the ground, and keep your elbow stable. Hold your chest out, lean forward slightly (right elbow does not leave the ground), the upper body naturally collapses, and the strength of your hands remains unchanged, so that the butt of the gun really leans on the shoulder socket, and your face leans forward slightly, naturally sticking to your cheeks.
According to the essentials of the gun, it can be summarized into ten words, namely: positive, grip, arrival, determination, collapse, no top and no pull.
Front: the right side of the gun body is basically aligned with the gun body, and the gun face should be front.
Grip method: Hold the right hand tightly, keep the grip strength unchanged when firing, and retract, fold and hold the right wrist.
Arrival: abutment shoulder position is suitable and tight.
Fixed: the elbow should be fixed.
Collapse: the upper body naturally collapses, keeping the gun posture stable.
Don't push or pull: it means to keep the force unchanged after the gun is pressed. It is forbidden to pull the gun backwards and push the shoulder forward.
Third, aim.
According to the gun, the upper edge of the notch, the upper edge of the sight and the lower edge of the target should be arranged in the same straight line. If you don't point at the target, you can't bend your body or change the action of the gun. You should adjust your posture or correct your support. You can move your body or elbow left and right when correcting. When correcting the height, you can move your body back and forth or move your elbow inside and outside, or you can adjust your support appropriately. When aiming, keep your eyes on the target. If you stare at the scope, it may weaken the target. In your line of sight, the line of sight should be a black shadow with clear and sharp edges.
Fourth, fire.
The first joint of the index finger of the right hand presses the trigger back evenly and upright (there should be a small gap between the inside of the index finger and the gun), and the strength of the other fingers remains unchanged. When the aiming line approaches the aiming point, start to preload the trigger and slow down your breathing. When the aiming line points to the aiming point, stop breathing and continue to increase the pressure of the trigger until firing. Correct and consistent aiming should be maintained when shooting. If the aiming line deviates from the aiming point or can't stop breathing, the pressure on the trigger can't be increased or relaxed, and then continue to hold the trigger after correction or ventilation. Until the shot went off and the shooting was completed. This is what is commonly said: "fire consciously, fire unconsciously."
5. Problems that are easy to exist.
1. Bang machine. It is easy to cause the gun body to swing, and the projectiles tend to lean to the left or miss the target, resulting in boring shots, which is caused by the shooter's tight grasp of the aiming point. This is the weakness caused by a failed shooter.
2. The firing timing is not good. The performance is either too early or too late, which often misses the opportunity, and more people hesitate to light it.
In addition, when shooting, we should pay attention to the relationship between the line of sight and the gap, and always keep the line of sight and the gap even, that is to say, when shooting, we should look at the line of sight, the gap is clear and the target is blurred.
These are just some basic shooting essentials. There are many reasons that affect the shooting accuracy, including wind, sunshine and so on, but it can be overcome as long as the correct shooting essentials are mastered.
What are the precautions in the notice? Notification is a document that a superior informs a subordinate about people and things. The circular is widely used by party and government organs and units at all levels. Its function is to praise good deeds, criticize mistakes and bad styles, report vicious accidents that should be taken as a warning, and convey important information and matters that all units need to know. Its purpose is to exchange experiences, learn lessons, educate cadres and workers and promote the further development of the work. The text structure of the commendation (criticism) notice has three parts: the first part explains the reasons for commendation or criticism, that is, it clearly describes the course of advanced deeds or wrong facts, and requires that the facts be truly and objectively reflected by narrative methods; The second part, make an accurate analysis and pertinent evaluation of the described facts, without exaggerating or narrowing them, so that people are encouraged by good people and things and learn from their mistakes; The third part, in general, is to reward or punish the advanced or criticize the mistakes. Finally, according to the reported situation and actual needs, issue a call or request. The text structure of the briefing generally has two parts: one is the situation of the briefing, and the other is the hope and requirement.