How to configure hybridization buffer for nucleic acid hybridization

The buffer names of SSC nucleic acid hybridization are all checked and prepared.

20X SSC comparison preparation:

Dosage: 1L

Preparatory work:

1. Accurately weigh175.2g of sodium chloride.

2. Accurately weigh 88.2g of sodium citrate.

3. Dissolve in 800 ml of water.

4. Add a few drops of 10mol/L sodium hydroxide solution and adjust the pH value to 7.0.

5. add water to constant volume to1l.

Note: 10×SSC, 5×SSC and 1×SSC were autoclaved according to the experimental requirements and diluted with 20×SSC.